Hedgehog stimulates maturation of Cubitus interruptus into a labile transcriptional activator

Johanna Talavera Ohlmeyer and Daniel Kalderon ()
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Johanna Talavera Ohlmeyer: Columbia University
Daniel Kalderon: Columbia University

Nature, 1998, vol. 396, issue 6713, 749-753

Abstract: Abstract In Drosophila, signalling by the protein Hedgehog (Hh) alters the activity of the transcription factor Cubitus interruptus (Ci) by inhibiting the proteolysis of full-length Ci (Ci-155) to its shortened Ci-75 form1,2. Ci-75 is found largely in the nucleus and isthought to be a transcriptional repressor1, whereas there is evidence3,4,5 to indicate that Ci-155 may be a transcriptional activator1,2,6. However, Ci-155 is detected only in the cytoplasm, where it is associated with the protein kinase Fused (Fu), with Suppressor of Fused (Su(fu)), and with the microtubule-binding protein Costal-2 (refs 1,7,8,9). It is not clear how Ci-155 might become a nuclear activator. We show here that mutations in Su ( fu) cause an increase in the expression of Hh-target genes in a dose-dependent manner while simultaneously reducing Ci-155 concentration by some mechanism other than proteolysis to Ci-75. Conversely, eliminating Fu kinase activity reduces Hh-target gene expression while increasing Ci-155 concentration. We propose that Fu kinase activity is required for Hh to stimulate the maturation of Ci-155 into a short-lived nuclear transcriptional activator and that Su(fu) opposes this maturation step through a stoichiometric interaction with Ci-155.

Date: 1998
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DOI: 10.1038/25533

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