Nuclear localization of Cdc25 is regulated by DNA damage and a 14-3-3 protein

Antonia Lopez-Girona, Beth Furnari, Odile Mondesert and Paul Russell ()
Additional contact information
Odile Mondesert: The Scripps Research Institute
Paul Russell: The Scripps Research Institute

Nature, 1999, vol. 397, issue 6715, 172-175

Abstract: Abstract DNA damage activates a cell-cycle checkpoint that prevents mitosis while DNA repair is under way1. The protein Chk1 enforces this checkpoint by phosphorylating the mitotic inducer Cdc25 (2–6). Phosphorylation of Cdc25 by Chk1 creates a binding site in Cdc25 for 14-3-3 proteins5,6,7,8, but it is not known how 14-3-3 proteins regulate Cdc25. Rad24 is a 14-3-3 protein that is important in the DNA-damage checkpoint in fission yeast9. Here we show that Rad24 controls the intracellular distribution of Cdc25. Elimination of Rad24 causes nuclear accumulation of Cdc25. Activation of the DNA-damage checkpoint causes the net nuclear export of Cdc25 by a process that requires Chk1, Rad24 and nuclear-export machinery. Mutation of a putative nuclear-export signal in Rad24 impairs the nuclear exclusion of Rad24, the damage-induced nuclear export of Cdc25 and the damage checkpoint. Thus, Rad24 appears to function as an attachable nuclear-export signal that enhances the nuclear export of Cdc25 in response to DNA damage.

Date: 1999
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/16488 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:397:y:1999:i:6715:d:10.1038_16488

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/16488

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().