Template-imprinted nanostructured surfaces for protein recognition

Huaiqiu Shi, Wei-Bor Tsai, Michael D. Garrison, Sandro Ferrari and Buddy D. Ratner ()
Additional contact information
Huaiqiu Shi: University of Washington
Wei-Bor Tsai: University of Washington
Michael D. Garrison: University of Washington
Sandro Ferrari: Ingengneria Dei Materiali, Universit Degli Studi Di Trento
Buddy D. Ratner: University of Washington

Nature, 1999, vol. 398, issue 6728, 593-597

Abstract: Abstract Synthetic materials capable of selectively recognizing proteins are important in separations1, biosensors2 and the development of biomedical materials3,4,5. The technique of molecular imprinting creates specific recognition sites in polymers by using template molecules6,7,8,9. Molecular recognition is attributed to binding sites that complement molecules in size, shape and chemical functionality10. But attempts to imprint proteins have met with only limited success11,12,13,14,15. Here we report a method for imprinting surfaces with protein-recognition sites. We use radio-frequency glow-discharge plasma deposition to form polymeric thin films16 around proteins coated with disaccharide molecules. The disaccharides become covalently attached to the polymer film, creating polysaccharide-like cavities that exhibit highly selective recognition for a variety of template proteins, including albumin, immunoglobulin G, lysozyme, ribonuclease and streptavidin. Direct imaging of template recognition is achieved by patterning a surface at the micrometre scale with imprinted regions.

Date: 1999
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/19267 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:398:y:1999:i:6728:d:10.1038_19267

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/19267

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().