Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase

Carlo Cogoni and Giuseppe Macino ()
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Carlo Cogoni: Sezione di Genetica Molecolare, Universita' di Roma La Sapienza, Viale Regina Elena 324
Giuseppe Macino: Sezione di Genetica Molecolare, Universita' di Roma La Sapienza, Viale Regina Elena 324

Nature, 1999, vol. 399, issue 6732, 166-169

Abstract: Abstract In plants and fungi, the introduction of transgenes can lead to post-transcriptional gene silencing1,2. This phenomenon, in which expression of the transgene and of endogenous genes containing sequences homologous to the transgene can be blocked, is involved in virus resistance3,4,5 and genome maintenance6,7. Transgene-induced gene silencing has been termed quelling in Neurospora crassa and co-suppression in plants. Quelling-defective (qde) mutants of N. crassa, in which transgene-induced gene silencing is impaired, have been isolated8. Here we report the cloning of qde-1, the first cellular component of the gene-silencing mechanism to be isolated, which defines a new gene family conserved among different species including plants, animals and fungi. The qde-1 gene product is similar to an RNA-dependent RNA polymerase found in the tomato9. The identification of qde-1 strongly supports models that implicate an RNA-dependent RNA polymerase in the post-transcriptional gene-silencing mechanism. The presence of qde-1 homologues in a variety of species of plants and fungi indicates that a conserved gene-silencing mechanism may exist, which could have evolved to preserve genome integrity and to protect the genome against naturally occurring transposons and viruses.

Date: 1999
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DOI: 10.1038/20215

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