Interaction of E1 and hSNF5 proteins stimulates replication of human papillomavirus DNA

Daeyoup Lee, Hekwang Sohn, Ganjam V. Kalpana, Joonho Choe and Joonho Choe ()
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Daeyoup Lee: Korea Advanced Institute of Science and Technology
Hekwang Sohn: Korea Advanced Institute of Science and Technology
Ganjam V. Kalpana: Albert Einstein College of Medicine
Joonho Choe: Korea Advanced Institute of Science and Technology
Joonho Choe: Korea Advanced Institute of Science and Technology

Nature, 1999, vol. 399, issue 6735, 487-491

Abstract: Abstract Mammalian viruses often use components of the host's cellular DNA replication machinery to carry out replication of their genomes, which enables these viruses to be used as tools for characterizing factors that are involved in cellular DNA replication. The human papillomavirus (HPV) E1 protein is essential for replication of the virus DNA1,2,3. Here we identify the cellular factor that participates in viral DNA replication by using a two-hybrid assay4 in the yeast Saccharomyces cerevisiae and E1 protein as bait. Using this assay, we isolated Ini1/hSNF5 (ref. 5), a component of the SWI/SNF complex which facilitates transcription by altering the structure of chromatin6. In vitro binding and immunoprecipitation confirmed that E1 interacts directly with Ini1/hSNF5. Transient DNA-replication assay revealed that HPV DNA replication is stimulated in a dose-dependent manner by addition of Ini1/hSNF5, and that Ini1/hSNF5 antisense RNA blocks the replication of HPV DNA. Amino-acid substitution at residues that are conserved among E1 proteins prevented the E1-Ini1/hSNF5 interaction and reduced DNA replication of HPV in vivo. Our results indicate that Ini1/hSNF5 is required for the efficient replication of papillomavirus DNA and is therefore needed, either alone or in complex with SWI/SNF complex, for mammalian DNA replication as well.

Date: 1999
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DOI: 10.1038/20966

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