Regions of variant histone His2AvD required for Drosophila development

Michael John Clarkson, Julian R. E. Wells, Frank Gibson, Robert Saint () and David John Tremethick ()
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Michael John Clarkson: The John Curtin School of Medical Research, the Australian National University
Julian R. E. Wells: Department of Biochemistry
Frank Gibson: The John Curtin School of Medical Research, the Australian National University
Robert Saint: University of Adelaide
David John Tremethick: The John Curtin School of Medical Research, the Australian National University

Nature, 1999, vol. 399, issue 6737, 694-697

Abstract: Abstract One way in which a distinct chromosomal domain could be established to carry out a specialized function is by the localized incorporation of specific histone variants into nucleosomes. H2AZ, one such variant of the histone protein H2A, is required for the survival of Drosophila melanogaster1, Tetrahymena thermophila2 and mice (R. Faast et al., in preparation). To search for the unique features of Drosophila H2AZ (His2AvD, also referred to as H2AvD) that are required for its essential function, we have performed amino-acid swap experiments in which residues unique to Drosophila His2AvD were replaced with equivalently positioned Drosophila H2A.1 residues. Mutated His2AvD genes encoding modified versions of this histone were transformed into Drosophila and tested for their ability to rescue null-mutant lethality. We show that the unique feature of His2AvD does not reside in its histone fold but in its carboxy-terminal domain. This C-terminal region maps to a short α-helix in H2A that is buried deep inside the nucleosome core.

Date: 1999
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DOI: 10.1038/21436

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