Nucleosome mobilization catalysed by the yeast SWI/SNF complex

Iestyn Whitehouse, Andrew Flaus, Bradley R. Cairns, Malcolm F. White, Jerry L. Workman and Tom Owen-Hughes ()
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Iestyn Whitehouse: The Wellcome Trust Building
Andrew Flaus: The Wellcome Trust Building
Bradley R. Cairns: Huntsman Cancer Institute
Malcolm F. White: University of Dundee
Jerry L. Workman: Howard Hughes Medical Institute, The Pennsylvania State University
Tom Owen-Hughes: The Wellcome Trust Building

Nature, 1999, vol. 400, issue 6746, 784-787

Abstract: Abstract The generation of a local chromatin topology conducive to transcription is a key step in gene regulation1. The yeast SWI/SNF complex is the founding member of a family of ATP-dependent remodelling activities capable of altering chromatin structure both in vitro and in vivo2. Despite its importance, the pathway by which the SWI/SNF complex disrupts chromatin structure is unknown. Here we use a model system to demonstrate that the yeast SWI/SNF complex can reposition nucleosomes in an ATP-dependent reaction that favours attachment of the histone octamer to an acceptor site on the same molecule of DNA (in cis). We show that SWI/SNF-mediated displacement of the histone octamer is effectively blocked by a barrier introduced into the DNA, suggesting that this redistribution involves sliding or tracking of nucleosomes along DNA, and that it is achieved by a catalytic mechanism. We conclude that SWI/SNF catalyses the redistribution of nucleosomes along DNA in cis, which may represent a general mechanism by which ATP-dependent chromatin remodelling occurs.

Date: 1999
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DOI: 10.1038/23506

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