The RNA splicing factor hSlu7 is required for correct 3′ splice-site choice

Katrin Chua and Robin Reed ()
Additional contact information
Katrin Chua: Harvard Medical School
Robin Reed: Harvard Medical School

Nature, 1999, vol. 402, issue 6758, 207-210

Abstract: Abstract The production of correctly spliced messenger RNA requires two catalytic splicing steps1,2,3. During step II, exon 1 attacks an adenine-guanine (AG) dinucleotide at the 3′ splice site. This AG is usually located between 18 and 40 nucleotides downstream from the branch site, and closer AGs are skipped in favour of AGs located more optimally downstream4,5,6. At present, little is understood about how the correct AG is distinguished from other AGs. Here we describe a metazoan splicing factor (hSlu7) that is required for selection of the correct AG. In the absence of hSlu7, use of the correct AG is suppressed and incorrect AGs are activated. We investigated this loss of fidelity by analysing spliceosomes assembled in the absence of hSlu7. These studies reveal that exon 1 is loosely associated with these spliceosomes. Thus, the improperly held exon cannot access the correct AG, but can attack other AGs indiscriminately. We conclude that hSlu7 is required to hold exon 1 tightly within the spliceosome for attack on a prespecified AG.

Date: 1999
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/46086 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:402:y:1999:i:6758:d:10.1038_46086

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/46086

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().