Chromosomal landscape of nucleosome-dependent gene expression and silencing in yeast

John J. Wyrick, Frank C. P. Holstege, Ezra G. Jennings, Helen C. Causton, David Shore, Michael Grunstein, Eric S. Lander and Richard A. Young ()
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John J. Wyrick: Whitehead Institute for Biomedical Research, Nine Cambridge Center
Frank C. P. Holstege: Whitehead Institute for Biomedical Research, Nine Cambridge Center
Ezra G. Jennings: Whitehead Institute for Biomedical Research, Nine Cambridge Center
Helen C. Causton: Whitehead Institute for Biomedical Research, Nine Cambridge Center
David Shore: University of Geneva
Michael Grunstein: UCLA School of Medicine and Molecular Biology Institute, University of California
Eric S. Lander: Whitehead Institute for Biomedical Research, Nine Cambridge Center
Richard A. Young: Massachusetts Institute of Technology

Nature, 1999, vol. 402, issue 6760, 418-421

Abstract: Abstract Eukaryotic genomes are packaged into nucleosomes, which are thought to repress gene expression generally1,2,3. Repression is particularly evident at yeast telomeres, where genes within the telomeric heterochromatin appear to be silenced by the histone-binding silent information regulator (SIR) complex (Sir2, Sir3, Sir4) and Rap1 (refs 4,5,6,7,8,9,10). Here, to investigate how nucleosomes and silencing factors influence global gene expression, we use high-density arrays to study the effects of depleting nucleosomal histones and silencing factors in yeast. Reducing nucleosome content by depleting histone H4 caused increased expression of 15% of genes and reduced expression of 10% of genes, but it had little effect on expression of the majority (75%) of yeast genes. Telomere-proximal genes were found to be de-repressed over regions extending 20 kilobases from the telomeres, well beyond the extent of Sir protein binding11,12 and the effects of loss of Sir function. These results indicate that histones make Sir-independent contributions to telomeric silencing, and that the role of histones located elsewhere in chromosomes is gene specific rather than generally repressive.

Date: 1999
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DOI: 10.1038/46567

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