Low fidelity DNA synthesis by human DNA polymerase-η

Toshiro Matsuda, Katarzyna Bebenek, Chikahide Masutani, Fumio Hanaoka and Thomas A. Kunkel ()
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Toshiro Matsuda: Laboratory of Molecular Genetics
Katarzyna Bebenek: Laboratory of Molecular Genetics
Chikahide Masutani: Institute for Molecular and Cellular Biology, Osaka University and CREST, Japan Science and Technology Corporation, 1-3 Yamada-oka
Fumio Hanaoka: Institute for Molecular and Cellular Biology, Osaka University and CREST, Japan Science and Technology Corporation, 1-3 Yamada-oka
Thomas A. Kunkel: Laboratory of Molecular Genetics

Nature, 2000, vol. 404, issue 6781, 1011-1013

Abstract: Abstract A superfamily of DNA polymerases that bypass lesions in DNA has been described1,2,3,4. Some family members are described as error-prone because mutations that inactivate the polymerase reduce damage-induced mutagenesis. In contrast, mutations in the skin cancer susceptibility gene XPV5,6, which encodes DNA polymerase (pol)-η, lead to increased ultraviolet-induced mutagenesis7,8,9,10,11. This, and the fact that pol-η primarily inserts adenines during efficient bypass of thymine–thymine dimers in vitro8,12,13, has led to the description of pol-η as error-free. However, here we show that human pol-η copies undamaged DNA with much lower fidelity than any other template-dependent DNA polymerase studied. Pol-η lacks an intrinsic proofreading exonuclease activity and, depending on the mismatch, makes one base substitution error for every 18 to 380 nucleotides synthesized. This very low fidelity indicates a relaxed requirement for correct base pairing geometry and indicates that the function of pol-η may be tightly controlled to prevent potentially mutagenic DNA synthesis.

Date: 2000
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DOI: 10.1038/35010014

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