Structural insights into the stereochemistry of the cyclooxygenase reaction

James R. Kiefer, Jennifer L. Pawlitz, Kirby T. Moreland, Roderick A. Stegeman, William F. Hood, James K. Gierse, Anna M. Stevens, Douglas C. Goodwin, Scott W. Rowlinson, Lawrence J. Marnett, William C. Stallings and Ravi G. Kurumbail ()
Additional contact information
James R. Kiefer: Searle Discovery Research, Monsanto Company
Jennifer L. Pawlitz: Searle Discovery Research, Monsanto Company
Kirby T. Moreland: Searle Discovery Research, Monsanto Company
Roderick A. Stegeman: Searle Discovery Research, Monsanto Company
William F. Hood: Searle Discovery Research, Monsanto Company
James K. Gierse: Searle Discovery Research, Monsanto Company
Anna M. Stevens: Searle Discovery Research, Monsanto Company
Douglas C. Goodwin: Vanderbilt School of Medicine, Vanderbilt University
Scott W. Rowlinson: Vanderbilt School of Medicine, Vanderbilt University
Lawrence J. Marnett: Vanderbilt School of Medicine, Vanderbilt University
William C. Stallings: Searle Discovery Research, Monsanto Company
Ravi G. Kurumbail: Searle Discovery Research, Monsanto Company

Nature, 2000, vol. 405, issue 6782, 97-101

Abstract: Abstract Cyclooxygenases are bifunctional enzymes that catalyse the first committed step in the synthesis of prostaglandins, thromboxanes and other eicosanoids1,2,3. The two known cyclooxygenases isoforms share a high degree of amino-acid sequence similarity1,2,3,4, structural topology5,6,7 and an identical catalytic mechanism1,2,3. Cyclooxygenase enzymes catalyse two sequential reactions in spatially distinct, but mechanistically coupled active sites8,9,10,11. The initial cyclooxygenase reaction converts arachidonic acid (which is achiral) to prostaglandin G2 (which has five chiral centres). The subsequent peroxidase reaction reduces prostaglandin G2 to prostaglandin H2. Here we report the co-crystal structures of murine apo-cyclooxygenase-2 in complex with arachidonic acid and prostaglandin. These structures suggest the molecular basis for the stereospecificity of prostaglandin G2 synthesis.

Date: 2000
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DOI: 10.1038/35011103

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