Transfection of cells by immunoporation

Lale Bildirici, Patricia Smith, Christos Tzavelas, Elina Horefti and David Rickwood ()
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Lale Bildirici: University of Essex
Patricia Smith: University of Essex
Christos Tzavelas: University of Essex
Elina Horefti: University of Essex
David Rickwood: University of Essex

Nature, 2000, vol. 405, issue 6784, 298-298

Abstract: Abstract Cell transfection is now a central technique in molecular biology and an essential prerequisite for gene therapy. Here we describe how beads coated with antibodies and bound to specific cell-surface transmembrane proteins can create holes in cells when the beads are removed, allowing transfection of the cells with DNA or other macromolecules. This unique targeted transfection of cells by immunoporation is very efficient and results in minimal cell death.

Date: 2000
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DOI: 10.1038/35012701

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