An electroneutral sodium/bicarbonate cotransporter NBCn1 and associated sodium channel

Inyeong Choi, Christian Aalkjaer, Emile L. Boulpaep and Walter F. Boron ()
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Inyeong Choi: Yale University School of Medicine
Christian Aalkjaer: University of Aarhus
Emile L. Boulpaep: Yale University School of Medicine
Walter F. Boron: Yale University School of Medicine

Nature, 2000, vol. 405, issue 6786, 571-575

Abstract: Abstract Two electroneutral, Na+-driven HCO-3 transporters, the Na+-driven Cl-/HCO-3 exchanger and the electroneutral Na+/ HCO-3 cotransporter, have crucial roles in regulating intracellular pH in a variety of cells, including cardiac myocytes1,2, vascular smooth-muscle3,4, neurons5 and fibroblasts6; however, it is difficult to distinguish their Cl- dependence in mammalian cells. Here we report the cloning of three variants of an electroneutral Na+/HCO-3 cotransporter, NBCn1, from rat smooth muscle. They are 89–92% identical to a human skeletal muscle clone7, 55–57% identical to the electrogenic NBCs and 33–43% identical to the anion exchangers8. When expressed in Xenopus oocytes, NBCn1-B (which encodes 1,218 amino acids) is electroneutral, Na+-dependent and HCO-3-dependent, but not Cl--dependent. Oocytes injected with low levels of NBCn1-B complementary RNA exhibit a Na+ conductance that 4,4-diisothiocyanatostilbene-2,2′-disulphonate stimulates slowly and irreversibly.

Date: 2000
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DOI: 10.1038/35014615

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