Cloned pigs produced by nuclear transfer from adult somatic cells

Irina A. Polejaeva (), Shu-Hung Chen, Todd D. Vaught, Raymond L. Page, June Mullins, Suyapa Ball, Yifan Dai, Jeremy Boone, Shawn Walker, David L. Ayares, Alan Colman and Keith H. S. Campbell
Additional contact information
Irina A. Polejaeva: PPL Therapeutics Incorporated
Shu-Hung Chen: PPL Therapeutics Incorporated
Todd D. Vaught: PPL Therapeutics Incorporated
Raymond L. Page: PPL Therapeutics Incorporated
June Mullins: PPL Therapeutics Incorporated
Suyapa Ball: PPL Therapeutics Incorporated
Yifan Dai: PPL Therapeutics Incorporated
Jeremy Boone: PPL Therapeutics Incorporated
Shawn Walker: PPL Therapeutics Incorporated
David L. Ayares: PPL Therapeutics Incorporated
Alan Colman: PPL Therapeutics
Keith H. S. Campbell: PPL Therapeutics

Nature, 2000, vol. 407, issue 6800, 86-90

Abstract: Abstract Since the first report of live mammals produced by nuclear transfer from a cultured differentiated cell population in 1995 (ref. 1), successful development has been obtained in sheep2,3, cattle4, mice5 and goats6 using a variety of somatic cell types as nuclear donors. The methodology used for embryo reconstruction in each of these species is essentially similar: diploid donor nuclei have been transplanted into enucleated MII oocytes that are activated on, or after transfer. In sheep2 and goat6 pre-activated oocytes have also proved successful as cytoplast recipients. The reconstructed embryos are then cultured and selected embryos transferred to surrogate recipients for development to term. In pigs, nuclear transfer has been significantly less successful; a single piglet was reported after transfer of a blastomere nucleus from a four-cell embryo to an enucleated oocyte7; however, no live offspring were obtained in studies using somatic cells such as diploid or mitotic fetal fibroblasts as nuclear donors8,9. The development of embryos reconstructed by nuclear transfer is dependent upon a range of factors. Here we investigate some of these factors and report the successful production of cloned piglets from a cultured adult somatic cell population using a new nuclear transfer procedure.

Date: 2000
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DOI: 10.1038/35024082

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