Total silencing by intron-spliced hairpin RNAs

Neil A. Smith, Surinder P. Singh, Ming-Bo Wang, Peter A. Stoutjesdijk, Allan G. Green and Peter M. Waterhouse ()
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Neil A. Smith: CSIRO Plant Industry
Surinder P. Singh: CSIRO Plant Industry
Ming-Bo Wang: CSIRO Plant Industry
Peter A. Stoutjesdijk: CSIRO Plant Industry
Allan G. Green: CSIRO Plant Industry
Peter M. Waterhouse: CSIRO Plant Industry

Nature, 2000, vol. 407, issue 6802, 319-320

Abstract: Abstract Post-transcriptional gene silencing (PTGS), a sequence-specific RNA degradation mechanism inherent in many life-forms, can be induced in plants by transforming them with either antisense1 or co-suppression2 constructs, but typically this results in only a small proportion of silenced individuals. Here we show that gene constructs encoding intron-spliced RNA with a hairpin structure can induce PTGS with almost 100% efficiency when directed against viruses or endogenous genes. These constructs could prove valuable in reverse genetics, genomics, engineering of metabolic pathways and protection against pathogens.

Date: 2000
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DOI: 10.1038/35030305

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