Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA

Amy E. Pasquinelli, Brenda J. Reinhart, Frank Slack, Mark Q. Martindale, Mitzi I. Kuroda, Betsy Maller, David C. Hayward, Eldon E. Ball, Bernard Degnan, Peter Müller, Jürg Spring, Ashok Srinivasan, Mark Fishman, John Finnerty, Joseph Corbo, Michael Levine, Patrick Leahy, Eric Davidson and Gary Ruvkun
Additional contact information
Amy E. Pasquinelli: Harvard Medical School
Brenda J. Reinhart: Harvard Medical School
Frank Slack: Cellular and Developmental Biology, Yale University
Mark Q. Martindale: Kewalo Marine Lab, Pacific Biomedical Research Center, University of Hawaii
Mitzi I. Kuroda: Howard Hughes Medical Institute, Baylor College of Medicine
Betsy Maller: Cellular and Developmental Biology, Yale University
David C. Hayward: Research School of Biological Sciences, PO Box 475, Australian National University
Eldon E. Ball: Research School of Biological Sciences, PO Box 475, Australian National University
Bernard Degnan: Department of Zoology and Entomology University of Queensland
Peter Müller: University of Basel
Jürg Spring: University of Basel
Ashok Srinivasan: Massachusetts General Hospital
Mark Fishman: Massachusetts General Hospital
John Finnerty: Boston University
Joseph Corbo: University of California
Michael Levine: University of California
Patrick Leahy: California Institute of Technology
Eric Davidson: California Institute of Technology
Gary Ruvkun: Harvard Medical School

Nature, 2000, vol. 408, issue 6808, 86-89

Abstract: Abstract Two small RNAs regulate the timing of Caenorhabditis elegans development1,2. Transition from the first to the second larval stage fates requires the 22-nucleotide lin-4 RNA1,3,4, and transition from late larval to adult cell fates requires the 21-nucleotide let-7 RNA2. The lin-4 and let-7 RNA genes are not homologous to each other, but are each complementary to sequences in the 3′ untranslated regions of a set of protein-coding target genes that are normally negatively regulated by the RNAs1,2,5,6. Here we have detected let-7 RNAs of ∼21 nucleotides in samples from a wide range of animal species, including vertebrate, ascidian, hemichordate, mollusc, annelid and arthropod, but not in RNAs from several cnidarian and poriferan species, Saccharomyces cerevisiae, Escherichia coli or Arabidopsis. We did not detect lin-4 RNA in these species. We found that let-7 temporal regulation is also conserved: let-7 RNA expression is first detected at late larval stages in C. elegans and Drosophila , at 48 hours after fertilization in zebrafish, and in adult stages of annelids and molluscs. The let-7 regulatory RNA may control late temporal transitions during development across animal phylogeny.

Date: 2000
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/35040556 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:408:y:2000:i:6808:d:10.1038_35040556

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/35040556

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().