A transcription reinitiation intermediate that is stabilized by activator

Natalya Yudkovsky, Jeffrey A. Ranish and Steven Hahn ()
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Natalya Yudkovsky: The Fred Hutchinson Cancer Research Center, and Molecular and Cellular Biology Program, University of Washington
Jeffrey A. Ranish: The Fred Hutchinson Cancer Research Center, and Molecular and Cellular Biology Program, University of Washington
Steven Hahn: The Fred Hutchinson Cancer Research Center, and Molecular and Cellular Biology Program, University of Washington

Nature, 2000, vol. 408, issue 6809, 225-229

Abstract: Abstract High levels of gene transcription by RNA polymerase II depend on high rates of transcription initiation and reinitiation. Initiation requires recruitment of the complete transcription machinery to a promoter, a process facilitated by activators and chromatin remodelling factors. Reinitiation probably occurs through a different pathway1. After initiation, a subset of the transcription machinery remains at the promoter, forming a platform for assembly of a second transcription complex2,3,4. Here we describe the isolation of a reinitiation intermediate that includes transcription factors TFIID, TFIIA, TFIIH, TFIIE and Mediator. This intermediate can act as a scaffold for formation of a functional reinitiation complex. Formation of this scaffold is dependent on ATP and TFIIH. The scaffold is stabilized in the presence of the activator Gal4–VP16, but not Gal4–AH, suggesting a new role for some activators and Mediator in promoting high levels of transcription.

Date: 2000
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DOI: 10.1038/35041603

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