Integration of telomere sequences with the draft human genome sequence

H. C. Riethman (), Z. Xiang, S. Paul, E. Morse, X.-L. Hu, J. Flint, H.-C. Chi, D. L. Grady and R. K. Moyzis
Additional contact information
H. C. Riethman: The Wistar Institute
Z. Xiang: The Wistar Institute
S. Paul: The Wistar Institute
E. Morse: The Wistar Institute
X.-L. Hu: The Wistar Institute
J. Flint: Institute of Molecular Medicine, John Radcliffe Hospital
H.-C. Chi: College of Medicine, University of California
D. L. Grady: College of Medicine, University of California
R. K. Moyzis: College of Medicine, University of California

Nature, 2001, vol. 409, issue 6822, 948-951

Abstract: Abstract Telomeres are the ends of linear eukaryotic chromosomes. To ensure that no large stretches of uncharacterized DNA remain between the ends of the human working draft sequence and the ends of each chromosome, we would need to connect the sequences of the telomeres to the working draft sequence. But telomeres have an unusual DNA sequence composition and organization that makes them particularly difficult to isolate and analyse. Here we use specialized linear yeast artificial chromosome clones, each carrying a large telomere-terminal fragment of human DNA, to integrate most human telomeres with the working draft sequence. Subtelomeric sequence structure appears to vary widely, mainly as a result of large differences in subtelomeric repeat sequence abundance and organization at individual telomeres. Many subtelomeric regions appear to be gene-rich, matching both known and unknown expressed genes. This indicates that human subtelomeric regions are not simply buffers of nonfunctional ‘junk DNA’ next to the molecular telomere, but are instead functional parts of the expressed genome.

Date: 2001
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/35057180 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:409:y:2001:i:6822:d:10.1038_35057180

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/35057180

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().