Selectivity of chromatin-remodelling cofactors for ligand-activated transcription

Bryan Lemon, Carla Inouye, David S. King and Robert Tjian ()
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Bryan Lemon: Howard Hughes Medical Institute, 401 Barker Hall, University of California
Carla Inouye: Howard Hughes Medical Institute, 401 Barker Hall, University of California
David S. King: Howard Hughes Medical Institute, 401 Barker Hall, University of California
Robert Tjian: Howard Hughes Medical Institute, 401 Barker Hall, University of California

Nature, 2001, vol. 414, issue 6866, 924-928

Abstract: Abstract An array of regulatory protein and multi-subunit cofactors has been identified1 that directs eukaryotic gene transcription. However, establishing the specific functions of various related cofactors has been difficult owing to the limitations inherent in assaying transcription in animals and cells indirectly. Here we describe, using an integrated chromatin-dependent reconstituted transcription reaction, the purification and identification of a multi-subunit cofactor (PBAF)2 that is necessary for ligand-dependent transactivation by nuclear hormone receptors. A highly related cofactor, human SWI/SNF3, and the ISWI-containing chromatin-remodelling complex ACF4 both fail to potentiate transcription. We also show that transcriptional activation mediated by nuclear hormone receptors requires TATA-binding protein (TBP)-associated factors (TAFs) as well as the multi-subunit cofactors ARC5/CRSP6. These studies demonstrate functional selectivity amongst highly related complexes involved in gene regulation and help define a more complete set of factors and cofactors required to activate transcription.

Date: 2001
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DOI: 10.1038/414924a

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