Stimulated platelets use serotonin to enhance their retention of procoagulant proteins on the cell surface

George L. Dale (), Paul Friese, Peter Batar, Stephen Hamilton, Guy L. Reed, Kenneth W. Jackson, Kenneth J. Clemetson and Lorenzo Alberio
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George L. Dale: University of Oklahoma Health Sciences Center
Paul Friese: University of Oklahoma Health Sciences Center
Peter Batar: University of Oklahoma Health Sciences Center
Guy L. Reed: Cardiovascular Biology Laboratory, Harvard School of Public Health
Kenneth W. Jackson: University of Oklahoma Health Sciences Center
Kenneth J. Clemetson: Theodor Kocher Institute, University of Berne
Lorenzo Alberio: Theodor Kocher Institute, University of Berne

Nature, 2002, vol. 415, issue 6868, 175-179

Abstract: Abstract Activated platelets bind numerous adhesive and procoagulant proteins by receptor-mediated processes1. Although there is little evidence to suggest that these processes are heterogeneous in platelets, we previously found that platelets co-stimulated with collagen and thrombin express functional α-granule factor V only on a subpopulation of cells2. Here we show that these cells, referred to as ‘COAT-platelets’, bind additional α-granule proteins, including fibrinogen, von Willebrand factor, thrombospondin, fibronectin and α2-antiplasmin. These proteins are all transglutaminase substrates, and inhibitors of transglutaminase prevent the production of COAT-platelets. A synthetic transglutaminase substrate (CP15) also binds to COAT-platelets, and analysis by high performance liquid chromatography/mass spectrometry shows that a product is formed with a relative molecular mass (Mr) equal to CP15 plus 176. Serotonin, an abundant component of platelet-dense granules3, has an Mr of 176, and fibrinogen isolated from COAT-platelets contains covalently linked serotonin. Synthetic bovine serum albumin-(serotonin)6 binds selectively to COAT-platelets and also inhibits the retention of procoagulant proteins on COAT-platelets. These data indicate that COAT-platelets use serotonin conjugation to augment the retention of procoagulant proteins on their cell surface through an as yet unidentified serotonin receptor.

Date: 2002
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DOI: 10.1038/415175a

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