Glutamate-receptor-interacting protein GRIP1 directly steers kinesin to dendrites

Mitsutoshi Setou, Dae-Hyung Seog, Yosuke Tanaka, Yoshimitsu Kanai, Yosuke Takei, Masahiko Kawagishi and Nobutaka Hirokawa ()
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Mitsutoshi Setou: University of Tokyo
Dae-Hyung Seog: University of Tokyo
Yosuke Tanaka: University of Tokyo
Yoshimitsu Kanai: University of Tokyo
Yosuke Takei: University of Tokyo
Masahiko Kawagishi: University of Tokyo
Nobutaka Hirokawa: University of Tokyo

Nature, 2002, vol. 417, issue 6884, 83-87

Abstract: Abstract In cells, molecular motors operate in polarized sorting of molecules, although the steering mechanisms of motors remain elusive1. In neurons, the kinesin motor2 conducts vesicular transport such as the transport of synaptic vesicle components to axons3 and of neurotransmitter receptors to dendrites4, indicating that vesicles may have to drive the motor for the direction to be correct. Here we show that an AMPA (α-amino-3-hydroxy-5-methylisoxazole-4-propionate) receptor subunit—GluR2-interacting protein (GRIP1)—can directly interact and steer kinesin heavy chains to dendrites as a motor for AMPA receptors. As would be expected if this complex is functional, both gene targeting and dominant negative experiments of heavy chains of mouse kinesin showed abnormal localization of GRIP1. Moreover, expression of the kinesin-binding domain of GRIP1 resulted in accumulation of the endogenous kinesin predominantly in the somatodendritic area. This pattern was different from that generated by the overexpression of the kinesin-binding scaffold protein JSAP1 (JNK/SAPK-associated protein-1, also known as Mapk8ip3), which occurred predominantly in the somatoaxon area. These results indicate that directly binding proteins can determine the traffic direction of a motor protein.

Date: 2002
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DOI: 10.1038/nature743

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