Multiply infected spleen cells in HIV patients

Andreas Jung, Reinhard Maier, Jean-Pierre Vartanian, Gennady Bocharov, Volker Jung, Ulrike Fischer, Eckart Meese, Simon Wain-Hobson and Andreas Meyerhans ()
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Andreas Jung: University of the Saarland
Reinhard Maier: University of the Saarland
Jean-Pierre Vartanian: Unité de Rétrovirologie Moléculaire, Institut Pasteur
Gennady Bocharov: Institute of Numerical Mathematics, Russian Academy of Sciences
Volker Jung: University of the Saarland
Ulrike Fischer: University of the Saarland
Eckart Meese: University of the Saarland
Simon Wain-Hobson: Unité de Rétrovirologie Moléculaire, Institut Pasteur
Andreas Meyerhans: University of the Saarland

Nature, 2002, vol. 418, issue 6894, 144-144

Abstract: Abstract The genome of the human immunodeficiency virus is highly prone to recombination1,2,3, although it is not obvious whether recombinants arise infrequently or whether they are constantly being spawned but escape identification because of the massive and rapid turnover of virus particles4,5. Here we use fluorescence in situ hybridization to estimate the number of proviruses harboured by individual splenocytes from two HIV patients, and determine the extent of recombination by sequencing amplified DNA from these cells. We find an average of three or four proviruses per cell and evidence for huge numbers of recombinants and extensive genetic variation. Although this creates problems for phylogenetic analyses, which ignore recombination effects, the intracellular variation may help to broaden immune recognition.

Date: 2002
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DOI: 10.1038/418144a

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