NMR analysis of a 900K GroEL–GroES complex

Jocelyne Fiaux, Eric B. Bertelsen, Arthur L. Horwich () and Kurt Wüthrich ()
Additional contact information
Jocelyne Fiaux: Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Zürich
Eric B. Bertelsen: Yale School of Medicine
Arthur L. Horwich: Yale School of Medicine
Kurt Wüthrich: Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Zürich

Nature, 2002, vol. 418, issue 6894, 207-211

Abstract: Abstract Biomacromolecular structures with a relative molecular mass (Mr) of 50,000 to 100,000 (50K–100K) have been generally considered to be inaccessible to analysis by solution NMR spectroscopy. Here we report spectra recorded from bacterial chaperonin complexes ten times this size limit (up to Mr 900K) using the techniques of transverse relaxation-optimized spectroscopy and cross-correlated relaxation-enhanced polarization transfer1,2,3,4,5. These techniques prevent deterioration of the NMR spectra by the rapid transverse relaxation of the magnetization to which large, slowly tumbling molecules are otherwise subject. We tested the resolving power of these techniques by examining the isotope-labelled homoheptameric co-chaperonin GroES (Mr 72K), either free in solution or in complex with the homotetradecameric chaperonin GroEL (Mr 800K) or with the single-ring GroEL variant SR1 (Mr 400K). Most amino acids of GroES show the same resonances whether free in solution or in complex with chaperonin; however, residues 17–32 show large chemical shift changes on binding. These amino acids belong to a mobile loop region of GroES that forms contacts with GroEL6,7,8,9,10. This establishes the utility of these techniques for solution NMR studies that should permit the exploration of structure, dynamics and interactions in large macromolecular complexes.

Date: 2002
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/nature00860 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:418:y:2002:i:6894:d:10.1038_nature00860

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature00860

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().