APC-dependent proteolysis of the mitotic cyclin Clb2 is essential for mitotic exit

Ralph Wäsch and Frederick R. Cross ()
Additional contact information
Ralph Wäsch: The Rockefeller University
Frederick R. Cross: The Rockefeller University

Nature, 2002, vol. 418, issue 6897, 556-562

Abstract: Abstract Cyclin degradation is central to regulation of the cell cycle. Mitotic exit was proposed to require degradation of the S phase cyclin Clb5 by the anaphase-promoting complex1,2 activated by Cdc20 (APCCdc20)3. Furthermore, Clb5 degradation was thought to be necessary for effective dephosphorylation and activation of the APC regulatory subunit Cdh1 (also known as Hct1) and the cyclin-dependent kinase inhibitor Sic1 by the phosphatase Cdc14, allowing mitotic kinase inactivation and mitotic exit3,4,5,6,7. Here we show, however, that spindle disassembly and cell division occur without significant APCCdc20-mediated Clb5 degradation, as well as in the absence of both Cdh1 and Sic1. We find instead that destruction-box-dependent degradation of the mitotic cyclin Clb2 is essential for mitotic exit. APCCdc20 may be required for an essential early phase of Clb2 degradation, and this phase may be sufficient for most aspects of mitotic exit. Cdh1 and Sic1 may be required for further inactivation of Clb2–Cdk1, regulating cell size and the length of G1.

Date: 2002
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/nature00856 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:418:y:2002:i:6897:d:10.1038_nature00856

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature00856

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().