A chromatin remodelling complex that loads cohesin onto human chromosomes

Mohamed-Ali Hakimi, Daniel A. Bochar, John A. Schmiesing, Yuanshu Dong, Orr G. Barak, David W. Speicher, Kyoko Yokomori and Ramin Shiekhattar ()
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Mohamed-Ali Hakimi: The Wistar Institute
Daniel A. Bochar: The Wistar Institute
John A. Schmiesing: University of California
Yuanshu Dong: The Wistar Institute
Orr G. Barak: The Wistar Institute
David W. Speicher: The Wistar Institute
Kyoko Yokomori: University of California
Ramin Shiekhattar: The Wistar Institute

Nature, 2002, vol. 418, issue 6901, 994-998

Abstract: Abstract Nucleosomal DNA is arranged in a higher-order structure that presents a barrier to most cellular processes involving protein DNA interactions1. The cellular machinery involved in sister chromatid cohesion, the cohesin complex, also requires access to the nucleosomal DNA to perform its function in chromosome segregation2,3,4,5,6,7,8,9,10. The machineries that provide this accessibility are termed chromatin remodelling factors11. Here, we report the isolation of a human ISWI (SNF2h)-containing chromatin remodelling complex that encompasses components of the cohesin and NuRD complexes. We show that the hRAD21 subunit of the cohesin complex directly interacts with the ATPase subunit SNF2h. Mapping of hRAD21, SNF2h and Mi2 binding sites by chromatin immunoprecipitation experiments reveals the specific association of these three proteins with human DNA elements containing Alu sequences. We find a correlation between modification of histone tails and association of the SNF2h/cohesin complex with chromatin. Moreover, we show that the association of the cohesin complex with chromatin can be regulated by the state of DNA methylation. Finally, we present evidence pointing to a role for the ATPase activity of SNF2h in the loading of hRAD21 on chromatin.

Date: 2002
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DOI: 10.1038/nature01024

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