Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells

Hiroshi Tauchi (), Junya Kobayashi, Ken-ichi Morishima, Dik C. van Gent, Takahiro Shiraishi, Nicole S. Verkaik, Diana vanHeems, Emi Ito, Asako Nakamura, Eiichiro Sonoda, Minoru Takata, Shunichi Takeda, Shinya Matsuura and Kenshi Komatsu ()
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Hiroshi Tauchi: Ibaraki University
Junya Kobayashi: Hiroshima University
Ken-ichi Morishima: Hiroshima University
Dik C. van Gent: Erasmus University Rotterdam
Takahiro Shiraishi: Hiroshima University
Nicole S. Verkaik: Erasmus University Rotterdam
Diana vanHeems: Erasmus University Rotterdam
Emi Ito: Hiroshima University
Asako Nakamura: Hiroshima University
Minoru Takata: Kawasaki Medical School
Shinya Matsuura: Hiroshima University
Kenshi Komatsu: Hiroshima University

Nature, 2002, vol. 420, issue 6911, 93-98

Abstract: Abstract Double-strand breaks occur during DNA replication and are also induced by ionizing radiation. There are at least two pathways which can repair such breaks: non-homologous end joining and homologous recombination (HR). Although these pathways are essentially independent of one another, it is possible that the proteins Mre11, Rad50 and Xrs2 are involved in both pathways in Saccharomyces cerevisiae1. In vertebrate cells, little is known about the exact function of the Mre11–Rad50–Nbs1 complex in the repair of double-strand breaks because Mre11- and Rad50-null mutations are lethal2. Here we show that Nbs1 is essential for HR-mediated repair in higher vertebrate cells. The disruption of Nbs1 reduces gene conversion and sister chromatid exchanges, similar to other HR-deficient mutants3. In fact, a site-specific double-strand break repair assay showed a notable reduction of HR events following generation of such breaks in Nbs1-disrupted cells. The rare recombinants observed in the Nbs1-disrupted cells were frequently found to have aberrant structures, which possibly arise from unusual crossover events, suggesting that the Nbs1 complex might be required to process recombination intermediates.

Date: 2002
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DOI: 10.1038/nature01125

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