EconPapers    
Economics at your fingertips  
 

Role for a Drosophila Myb-containing protein complex in site-specific DNA replication

Eileen L. Beall, J. Robert Manak, Sharleen Zhou, Maren Bell, Joseph S. Lipsick and Michael R. Botchan ()
Additional contact information
Eileen L. Beall: University of California
J. Robert Manak: Stanford University School of Medicine
Sharleen Zhou: University of California
Maren Bell: University of California
Joseph S. Lipsick: Stanford University School of Medicine
Michael R. Botchan: University of California

Nature, 2002, vol. 420, issue 6917, 833-837

Abstract: Abstract There is considerable interest in the developmental, temporal and tissue-specific patterns of DNA replication in metazoans1,2. Site-specific DNA replication at the chorion loci in Drosophila follicle cells leads to extensive gene amplification, and the organization of the cis-acting DNA elements that regulate this process may provide a model for how such regulation is achieved3. Two elements important for amplification of the third chromosome chorion gene cluster, ACE3 and Ori-β, are directly bound by Orc4,5,6 (origin recognition complex), and two-dimensional gel analysis has revealed that the primary origin used is Ori-β (refs 7–9). Here we show that the Drosophila homologue of the Myb (Myeloblastosis) oncoprotein family is tightly associated with four additional proteins, and that the complex binds site-specifically to these regulatory DNA elements. Drosophila Myb is required in trans for gene amplification, showing that a Myb protein is directly involved in DNA replication. A Drosophila Myb binding site, as well as the binding site for another Myb complex member (p120), is necessary in cis for replication of reporter transgenes. Chromatin immunoprecipitation experiments localize both proteins to the chorion loci in vivo. These data provide evidence that specific protein complexes bound to replication enhancer elements work together with the general replication machinery for site-specific origin utilization during replication.

Date: 2002
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/nature01228 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:420:y:2002:i:6917:d:10.1038_nature01228

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature01228

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().

 
Page updated 2025-03-19
Handle: RePEc:nat:nature:v:420:y:2002:i:6917:d:10.1038_nature01228