Structure of the Escherichia coli ribosomal termination complex with release factor 2

Bruno P. Klaholz, Tillmann Pape, Andrey V. Zavialov, Alexander G. Myasnikov, Elena V. Orlova, Bente Vestergaard, Måns Ehrenberg and Marin van Heel ()
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Bruno P. Klaholz: Imperial College of Science, Technology and Medicine
Tillmann Pape: Imperial College of Science, Technology and Medicine
Andrey V. Zavialov: Uppsala University
Alexander G. Myasnikov: Imperial College of Science, Technology and Medicine
Elena V. Orlova: Imperial College of Science, Technology and Medicine
Bente Vestergaard: University of Aarhus
Måns Ehrenberg: Uppsala University
Marin van Heel: Imperial College of Science, Technology and Medicine

Nature, 2003, vol. 421, issue 6918, 90-94

Abstract: Abstract Termination of protein synthesis1 occurs when the messenger RNA presents a stop codon in the ribosomal aminoacyl (A) site. Class I release factor proteins (RF1 or RF2) are believed to recognize stop codons via tripeptide motifs2, leading to release of the completed polypeptide chain from its covalent attachment to transfer RNA in the ribosomal peptidyl (P) site. Class I RFs possess a conserved GGQ amino-acid motif that is thought to be involved directly in protein–transfer-RNA bond hydrolysis3,4. Crystal structures of bacterial and eukaryotic class I RFs have been determined5,6, but the mechanism of stop codon recognition and peptidyl-tRNA hydrolysis remains unclear. Here we present the structure of the Escherichia coli ribosome in a post-termination complex with RF2, obtained by single-particle cryo-electron microscopy (cryo-EM). Fitting the known 70S and RF2 structures into the electron density map reveals that RF2 adopts a different conformation on the ribosome when compared with the crystal structure of the isolated protein. The amino-terminal helical domain of RF2 contacts the factor-binding site of the ribosome, the ‘SPF’ loop of the protein is situated close to the mRNA, and the GGQ-containing domain of RF2 interacts with the peptidyl-transferase centre (PTC). By connecting the ribosomal decoding centre with the PTC, RF2 functionally mimics a tRNA molecule in the A site. Translational termination in eukaryotes is likely to be based on a similar mechanism.

Date: 2003
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DOI: 10.1038/nature01225

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