Sequence-specific recruitment of transcriptional co-repressor Cabin1 by myocyte enhancer factor-2

Aidong Han, Fan Pan, James C. Stroud, Hong-Duk Youn, Jun O. Liu and Lin Chen ()
Additional contact information
Aidong Han: University of Colorado at Boulder
Fan Pan: Johns Hopkins University School of Medicine
James C. Stroud: University of Colorado at Boulder
Hong-Duk Youn: Johns Hopkins University School of Medicine
Jun O. Liu: Johns Hopkins University School of Medicine
Lin Chen: University of Colorado at Boulder

Nature, 2003, vol. 422, issue 6933, 730-734

Abstract: Abstract The myocyte enhancer factor-2 (MEF2) family of transcription factors has important roles in the development and function of T cells, neuronal cells and muscle cells1,2,3. MEF2 is capable of repressing or activating transcription by association with a variety of co-repressors or co-activators in a calcium-dependent manner1,4,5. Transcriptional repression by MEF2 has attracted particular attention because of its potential role in hypertrophic responses of cardiomyocytes6. Several MEF2 co-repressors, such as Cabin1/Cain and class II histone deacetylases (HDACs), have been identified7,8,9,10,11,12. However, the molecular mechanism of their recruitment to specific promoters by MEF2 remains largely unknown. Here we report a crystal structure of the MADS-box/MEF2S domain of human MEF2B bound to a motif of the transcriptional co-repressor Cabin1 and DNA at 2.2 Å resolution. The crystal structure reveals a stably folded MEF2S domain on the surface of the MADS box. Cabin1 adopts an amphipathic α-helix to bind a hydrophobic groove on the MEF2S domain, forming a triple-helical interaction. Our studies of the ternary Cabin1/MEF2/DNA complex show a general mechanism by which MEF2 recruits transcriptional co-repressor Cabin1 and class II HDACs to specific DNA sites.

Date: 2003
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/nature01555 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:422:y:2003:i:6933:d:10.1038_nature01555

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature01555

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().