Three modes of synaptic vesicular recycling revealed by single-vesicle imaging

Sunil P. Gandhi and Charles F. Stevens ()
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Sunil P. Gandhi: University of California
Charles F. Stevens: Howard Hughes Medical Institute, and Molecular Neurobiology Laboratory, The Salk Institute

Nature, 2003, vol. 423, issue 6940, 607-613

Abstract: Abstract Synapses recycle their spent vesicles in order to keep up with on-going neurotransmitter release. To investigate vesicle recycling in the small synapses of hippocampal neurons, we have used an optical recording method that permits us to resolve single-vesicle events. Here we show that an exocytic event can terminate with three modes of vesicle retrieval: a fast (400–860 ms) ‘kiss-and-run’ mode that has a selective fusion pore; a slow (8–21 s) ‘compensatory’ mode; and a ‘stranded’ mode of recycling, in which a vesicle is left on the cell surface until a nerve impulse triggers its retrieval. We have also observed that, in response to a nerve impulse, synapses with low release probability primarily use the kiss-and-run mode, whereas high release probability terminals predominantly use the compensatory mode of vesicle retrieval.

Date: 2003
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DOI: 10.1038/nature01677

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