ER–phagosome fusion defines an MHC class I cross-presentation compartment in dendritic cells

Pierre Guermonprez, Loredana Saveanu, Monique Kleijmeer, Jean Davoust, Peter van Endert and Sebastian Amigorena ()
Additional contact information
Pierre Guermonprez: INSERM U520, Institut Curie
Loredana Saveanu: INSERM U580, Institut Necker
Monique Kleijmeer: UMC Utrecht
Jean Davoust: CNRS UMR8115, Genethon
Peter van Endert: INSERM U580, Institut Necker
Sebastian Amigorena: INSERM U520, Institut Curie

Nature, 2003, vol. 425, issue 6956, 397-402

Abstract: Abstract Induction of cytotoxic T-cell immunity requires the phagocytosis of pathogens, virus-infected or dead tumour cells by dendritic cells1. Peptides derived from phagocytosed antigens are then presented to CD8+ T lymphocytes on major histocompatibility complex (MHC) class I molecules, a process called “cross-presentation”2,3. After phagocytosis, antigens are exported into the cytosol and degraded by the proteasome4,5,6. The resulting peptides are thought to be translocated into the lumen of the endoplasmic reticulum (ER) by specific transporters associated with antigen presentation (TAP), and loaded onto MHC class I molecules by a complex “loading machinery” (which includes tapasin, calreticulin and Erp57)7. Here we show that soon after or during formation, phagosomes fuse with the ER. After antigen export to the cytosol and degradation by the proteasome, peptides are translocated by TAP into the lumen of the same phagosomes, before loading on phagosomal MHC class I molecules. Therefore, cross-presentation in dendritic cells occurs in a specialized, self-sufficient, ER–phagosome mix compartment.

Date: 2003
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/nature01911 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:425:y:2003:i:6956:d:10.1038_nature01911

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature01911

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().