A conspicuous nickel protein in microbial mats that oxidize methane anaerobically

Martin Krüger, Anke Meyerdierks, Frank Oliver Glöckner, Rudolf Amann, Friedrich Widdel (), Michael Kube, Richard Reinhardt, Jörg Kahnt, Reinhard Böcher, Rudolf K. Thauer () and Seigo Shima
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Martin Krüger: Max Planck Institute for Marine Microbiology
Anke Meyerdierks: Max Planck Institute for Marine Microbiology
Frank Oliver Glöckner: Max Planck Institute for Marine Microbiology
Rudolf Amann: Max Planck Institute for Marine Microbiology
Friedrich Widdel: Max Planck Institute for Marine Microbiology
Michael Kube: Max Planck Institute for Molecular Genetics, Ihnestraße
Richard Reinhardt: Max Planck Institute for Molecular Genetics, Ihnestraße
Jörg Kahnt: Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße
Reinhard Böcher: Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße
Rudolf K. Thauer: Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße
Seigo Shima: Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße

Nature, 2003, vol. 426, issue 6968, 878-881

Abstract: Abstract Anaerobic oxidation of methane (AOM) in marine sediments is an important microbial process in the global carbon cycle and in control of greenhouse gas emission. The responsible organisms supposedly reverse the reactions of methanogenesis1,2,3,4,5,6,7,8, but cultures providing biochemical proof of this have not been isolated. Here we searched for AOM-associated cell components in microbial mats from anoxic methane seeps in the Black Sea9,10,11. These mats catalyse AOM rather than carry out methanogenesis. We extracted a prominent nickel compound displaying the same absorption spectrum as the nickel cofactor F430 of methyl-coenzyme M reductase, the terminal enzyme of methanogenesis12; however, the nickel compound exhibited a higher molecular mass than F430. The apparent variant of F430 was part of an abundant protein that was purified from the mat and that consists of three different subunits. Determined amino-terminal amino acid sequences matched a gene locus cloned from the mat. Sequence analyses revealed similarities to methyl-coenzyme M reductase from methanogenic archaea. The abundance of the nickel protein (7% of extracted proteins) in the mat suggests an important role in AOM.

Date: 2003
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DOI: 10.1038/nature02207

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