T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases

Thorsten R. Mempel, Sarah E. Henrickson and Ulrich H. von Andrian ()
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Thorsten R. Mempel: Harvard Medical School
Sarah E. Henrickson: Harvard Medical School
Ulrich H. von Andrian: Harvard Medical School

Nature, 2004, vol. 427, issue 6970, 154-159

Abstract: Abstract Primary T-cell responses in lymph nodes (LNs) require contact-dependent information exchange between T cells and dendritic cells (DCs). Because lymphocytes continually enter and leave normal LNs, the resident lymphocyte pool is composed of non-synchronized cells with different dwell times that display heterogeneous behaviour in mouse LNs in vitro1,2,3. Here we employ two-photon microscopy in vivo to study antigen-presenting DCs and naive T cells whose dwell time in LNs was synchronized. During the first 8 h after entering from the blood, T cells underwent multiple short encounters with DCs, progressively decreased their motility, and upregulated activation markers. During the subsequent 12 h T cells formed long-lasting stable conjugates with DCs and began to secrete interleukin-2 and interferon-γ. On the second day, coinciding with the onset of proliferation, T cells resumed their rapid migration and short DC contacts. Thus, T-cell priming by DCs occurs in three successive stages: transient serial encounters during the first activation phase are followed by a second phase of stable contacts culminating in cytokine production, which makes a transition into a third phase of high motility and rapid proliferation.

Date: 2004
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DOI: 10.1038/nature02238

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