The MAPK Hog1 recruits Rpd3 histone deacetylase to activate osmoresponsive genes

Eulàlia de Nadal, Meritxell Zapater, Paula M. Alepuz, Lauro Sumoy, Glòria Mas and Francesc Posas ()
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Eulàlia de Nadal: Universitat Pompeu Fabra (UPF)
Meritxell Zapater: Universitat Pompeu Fabra (UPF)
Paula M. Alepuz: Universitat Pompeu Fabra (UPF)
Lauro Sumoy: Centre de Regulació Genòmica (CRG)
Glòria Mas: Universitat Pompeu Fabra (UPF)
Francesc Posas: Universitat Pompeu Fabra (UPF)

Nature, 2004, vol. 427, issue 6972, 370-374

Abstract: Abstract Regulation of gene expression by mitogen-activated protein kinases (MAPKs) is essential for proper cell adaptation to extracellular stimuli. Exposure of yeast cells to high osmolarity results in rapid activation of the MAPK Hog1, which coordinates the transcriptional programme required for cell survival on osmostress1. The mechanisms by which Hog1 and MAPKs in general regulate gene expression are not completely understood, although Hog1 can modify some transcription factors2. Here we propose that Hog1 induces gene expression by a mechanism that involves recruiting a specific histone deacetylase complex to the promoters of genes regulated by osmostress. Cells lacking the Rpd3–Sin3 histone deacetylase complex are sensitive to high osmolarity and show compromised expression of osmostress genes. Hog1 interacts physically with Rpd3 in vivo and in vitro and, on stress, targets the deacetylase to specific osmostress-responsive genes. Binding of the Rpd3–Sin3 complex to specific promoters leads to histone deacetylation, entry of RNA polymerase II and induction of gene expression. Together, our data indicate that targeting of the Rpd3 histone deacetylase to osmoresponsive promoters by the MAPK Hog1 is required to induce gene expression on stress.

Date: 2004
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DOI: 10.1038/nature02258

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