Identification of the pollen determinant of S-RNase-mediated self-incompatibility

Paja Sijacic, Xi Wang, Andrea L. Skirpan, Yan Wang, Peter E. Dowd, Andrew G. McCubbin, Shihshieh Huang and Teh-hui Kao ()
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Paja Sijacic: The Pennsylvania State University, 403 Althouse Laboratory
Xi Wang: The Pennsylvania State University, 403 Althouse Laboratory
Andrea L. Skirpan: The Pennsylvania State University, 403 Althouse Laboratory
Yan Wang: The Pennsylvania State University, 403 Althouse Laboratory
Peter E. Dowd: The Pennsylvania State University, 403 Althouse Laboratory
Andrew G. McCubbin: The Pennsylvania State University, 403 Althouse Laboratory
Shihshieh Huang: Mystic Research, Monsanto Company
Teh-hui Kao: The Pennsylvania State University, 403 Althouse Laboratory

Nature, 2004, vol. 429, issue 6989, 302-305

Abstract: Abstract Many flowering plants have adopted self-incompatibility mechanisms to prevent inbreeding and promote out-crosses1. In the Solanaceae, Rosaceae and Scrophulariaceae, two separate genes at the highly polymorphic S-locus control self-incompatibility interactions: the S-RNase gene encodes the pistil determinant and the previously unidentified S-gene encodes the pollen determinant2,3,4. S-RNases interact with pollen S-allele products to inhibit the growth of self-pollen tubes in the style. Pollen-expressed F-box genes showing allelic sequence polymorphism have recently been identified near to the S-RNase gene in members of the Rosaceae and Scrophulariaceae5,6,7,8; but until now have not been directly shown to encode the pollen determinant. Here we report the identification and characterization of PiSLF, an S-locus F-box gene of Petunia inflata (Solanaceae). We show that transformation of S1S1, S1S2 and S2S3 plants with the S2-allele of PiSLF causes breakdown of their pollen function in self-incompatibility. This breakdown of pollen function is consistent with ‘competitive interaction’, in which pollen carrying two different pollen S-alleles fails to function in self-incompatibility1,9,10. We conclude that PiSLF encodes the pollen self-incompatibility determinant.

Date: 2004
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DOI: 10.1038/nature02523

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