Replication by human DNA polymerase-ι occurs by Hoogsteen base-pairing

Deepak T. Nair, Robert E. Johnson, Satya Prakash, Louise Prakash and Aneel K. Aggarwal ()
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Deepak T. Nair: Mount Sinai School of Medicine
Robert E. Johnson: University of Texas Medical Branch
Satya Prakash: University of Texas Medical Branch
Louise Prakash: University of Texas Medical Branch
Aneel K. Aggarwal: Mount Sinai School of Medicine

Nature, 2004, vol. 430, issue 6997, 377-380

Abstract: Abstract Almost all DNA polymerases show a strong preference for incorporating the nucleotide that forms the correct Watson–Crick base pair with the template base. In addition, the catalytic efficiencies with which any given polymerase forms the four possible correct base pairs are roughly the same. Human DNA polymerase-ι (hPolι), a member of the Y family of DNA polymerases, is an exception to these rules. hPolι incorporates the correct nucleotide opposite a template adenine with a several hundred to several thousand fold greater efficiency than it incorporates the correct nucleotide opposite a template thymine, whereas its efficiency for correct nucleotide incorporation opposite a template guanine or cytosine is intermediate between these two extremes1,2,3,4,5. Here we present the crystal structure of hPolι bound to a template primer and an incoming nucleotide. The structure reveals a polymerase that is ‘specialized’ for Hoogsteen base-pairing, whereby the templating base is driven to the syn conformation. Hoogsteen base-pairing offers a basis for the varied efficiencies and fidelities of hPolι opposite different template bases, and it provides an elegant mechanism for promoting replication through minor-groove purine adducts that interfere with replication.

Date: 2004
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DOI: 10.1038/nature02692

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