Separase-mediated cleavage of cohesin at interphase is required for DNA repair

Koji Nagao, Yoh Adachi and Mitsuhiro Yanagida ()
Additional contact information
Koji Nagao: Graduate School of Science
Yoh Adachi: Kyoto University
Mitsuhiro Yanagida: Graduate School of Science

Nature, 2004, vol. 430, issue 7003, 1044-1048

Abstract: Abstract Sister chromatids are held together by cohesins1. At anaphase, separase is activated by degradation of its inhibitory partner, securin2,3. Separase then cleaves cohesins4,5,6, thus allowing sister chromatid separation. Fission yeast securin (Cut2) has destruction boxes and a separase (Cut1) interaction site in the amino and carboxyl terminus, respectively7,8. Here we show that securin is essential for separase stability and also for proper repair of DNA damaged by ultraviolet, X-ray and γ-ray irradiation. The cut2EA2 mutant is defective in the repair of ultraviolet damage lesions, although the DNA damage checkpoint is activated normally. In double mutant analysis of ultraviolet sensitivity, checkpoint kinase chk1 (ref. 9) and excision repair rad13 (ref. 10) mutants were additive with cut2EA2, whereas recombination repair rhp51 (ref. 11) and cohesin subunit rad21 (ref. 12) mutants were not. Cohesin was hyper-modified on ultraviolet irradiation in a Rad3 kinase-dependent way13. Experiments using either mutant cohesin that cannot be cleaved by separase or a protease-dead separase provide evidence that this DNA repair function of securin–separase acts through the cleavage of cohesin. We propose that the securin–separase complex might aid DNA repair by removing local cohesin in interphase cells.

Date: 2004
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/nature02803 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:430:y:2004:i:7003:d:10.1038_nature02803

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature02803

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().