Identification of human brain tumour initiating cells

Sheila K. Singh, Cynthia Hawkins, Ian D. Clarke, Jeremy A. Squire, Jane Bayani, Takuichiro Hide, R. Mark Henkelman, Michael D. Cusimano and Peter B. Dirks ()
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Sheila K. Singh: University of Toronto
Cynthia Hawkins: University of Toronto
Ian D. Clarke: University of Toronto
Jeremy A. Squire: Ontario Cancer Institute and University of Toronto
Jane Bayani: Ontario Cancer Institute and University of Toronto
Takuichiro Hide: University of Toronto
R. Mark Henkelman: The Hospital for Sick Children and University of Toronto
Michael D. Cusimano: University of Toronto
Peter B. Dirks: University of Toronto

Nature, 2004, vol. 432, issue 7015, 396-401

Abstract: Abstract The cancer stem cell (CSC) hypothesis suggests that neoplastic clones are maintained exclusively by a rare fraction of cells with stem cell properties1,2. Although the existence of CSCs in human leukaemia is established3,4, little evidence exists for CSCs in solid tumours, except for breast cancer5. Recently, we prospectively isolated a CD133+ cell subpopulation from human brain tumours that exhibited stem cell properties in vitro6. However, the true measures of CSCs are their capacity for self renewal and exact recapitulation of the original tumour1,2,7. Here we report the development of a xenograft assay that identified human brain tumour initiating cells that initiate tumours in vivo. Only the CD133+ brain tumour fraction contains cells that are capable of tumour initiation in NOD-SCID (non-obese diabetic, severe combined immunodeficient) mouse brains. Injection of as few as 100 CD133+ cells produced a tumour that could be serially transplanted and was a phenocopy of the patient's original tumour, whereas injection of 105 CD133- cells engrafted but did not cause a tumour. Thus, the identification of brain tumour initiating cells provides insights into human brain tumour pathogenesis, giving strong support for the CSC hypothesis as the basis for many solid tumours5, and establishes a previously unidentified cellular target for more effective cancer therapies.

Date: 2004
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DOI: 10.1038/nature03128

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