Human 5′ → 3′ exonuclease Xrn2 promotes transcription termination at co-transcriptional cleavage sites

Steven West, Natalia Gromak and Nick J. Proudfoot ()
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Steven West: University of Oxford
Natalia Gromak: University of Oxford
Nick J. Proudfoot: University of Oxford

Nature, 2004, vol. 432, issue 7016, 522-525

Abstract: Abstract Eukaryotic protein-encoding genes possess poly(A) signals that define the end of the messenger RNA and mediate downstream transcriptional termination by RNA polymerase II (Pol II)1. Termination could occur through an ‘anti-termination’ mechanism whereby elongation factors dissociate when the poly(A) signal is encountered, producing termination-competent Pol II2,3. An alternative ‘torpedo’ model postulated that poly(A) site cleavage provides an unprotected RNA 5′ end that is degraded by 5′ → 3′ exonuclease activities (torpedoes) and so induces dissociation of Pol II from the DNA template1,4. This model has been questioned because unprocessed transcripts read all the way to the site of transcriptional termination before upstream polyadenylation5,6,7. However, nascent transcripts located 1 kilobase downstream of the human β-globin gene poly(A) signal are associated with a co-transcriptional cleavage (CoTC) activity8 that acts with the poly(A) signal to elicit efficient transcriptional termination. The CoTC sequence is an autocatalytic RNA structure that undergoes rapid self-cleavage9. Here we show that CoTC acts as a precursor to termination by presenting a free RNA 5′ end that is recognized by the human 5′ → 3′ exonuclease Xrn2. Degradation of the downstream cleavage product by Xrn2 results in transcriptional termination, as envisaged in the torpedo model.

Date: 2004
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DOI: 10.1038/nature03035

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