The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II

Minkyu Kim, Nevan J. Krogan, Lidia Vasiljeva, Oliver J. Rando, Eduard Nedea, Jack F. Greenblatt and Stephen Buratowski ()
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Minkyu Kim: Harvard Medical School
Nevan J. Krogan: University of Toronto
Lidia Vasiljeva: Harvard Medical School
Oliver J. Rando: Harvard University
Eduard Nedea: University of Toronto
Jack F. Greenblatt: University of Toronto
Stephen Buratowski: Harvard Medical School

Nature, 2004, vol. 432, issue 7016, 517-522

Abstract: Abstract The carboxy-terminal domain (CTD) of the RNA polymerase II (RNApII) largest subunit consists of multiple heptapeptide repeats with the consensus sequence YSPTSPS. Different CTD phosphorylation patterns act as recognition sites for the binding of various messenger RNA processing factors, thereby coupling transcription and mRNA processing1. Polyadenylation factors are co-transcriptionally recruited by phosphorylation of CTD serine 2 (ref. 2) and these factors are also required for transcription termination3,4. RNApII transcribes past the poly(A) site, the RNA is cleaved by the polyadenylation machinery, and the RNA downstream of the cleavage site is degraded. Here we show that Rtt103 and the Rat1/Rai1 5′ → 3′ exonuclease are localized at 3′ ends of protein coding genes. In rat1-1 or rai1Δ cells, RNA 3′ to polyadenylation sites is greatly stabilized and termination defects are seen at many genes. These findings support a model in which poly(A) site cleavage and subsequent degradation of the 3′-downstream RNA by Rat1 trigger transcription termination5,6.

Date: 2004
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DOI: 10.1038/nature03041

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