Recruitment of Drosophila Polycomb group proteins to chromatin by DSP1

Jérôme Déjardin, Aurélien Rappailles, Olivier Cuvier, Charlotte Grimaud, Martine Decoville, Daniel Locker and Giacomo Cavalli ()
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Jérôme Déjardin: Institute of Human Genetics, CNRS
Aurélien Rappailles: Centre de Biophysique Moléculaire, CNRS/Université d'Orléans
Olivier Cuvier: Institute of Human Genetics, CNRS
Charlotte Grimaud: Institute of Human Genetics, CNRS
Martine Decoville: Centre de Biophysique Moléculaire, CNRS/Université d'Orléans
Daniel Locker: Centre de Biophysique Moléculaire, CNRS/Université d'Orléans
Giacomo Cavalli: Institute of Human Genetics, CNRS

Nature, 2005, vol. 434, issue 7032, 533-538

Abstract: Abstract Polycomb and trithorax group (PcG and trxG) proteins maintain silent and active transcriptional states, respectively, throughout development1. In Drosophila, PcG and trxG proteins associate with DNA regions named Polycomb and trithorax response elements (PRE and TRE), but the mechanisms of recruitment are unknown. We previously characterized a minimal element from the regulatory region of the Abdominal-B gene, termed Ab-Fab. Ab-Fab contains a PRE and a TRE and is able to maintain repressed or active chromatin states during development2. Here we show that the Dorsal switch protein 1 (DSP1), a Drosophila HMGB2 homologue, binds to a sequence present within Ab-Fab and in other characterized PREs. Addition of this motif to an artificial sequence containing Pleiohomeotic and GAGA factor consensus sites is sufficient for PcG protein recruitment in vivo. Mutations that abolish DSP1 binding to Ab-Fab and to a PRE from the engrailed locus lead to loss of PcG protein binding, loss of silencing, and switching of these PREs into constitutive TREs. The binding of DSP1 to PREs is therefore important for the recruitment of PcG proteins.

Date: 2005
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DOI: 10.1038/nature03386

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