Hoogsteen base-pairing in DNA replication?

Jimin Wang ()
Additional contact information
Jimin Wang: Center for Structural Biology, Yale University

Nature, 2005, vol. 437, issue 7057, E6-E7

Abstract: Abstract Arising from: D. T. Nair, R. E. Johnson, S. Prakash, L. Prakash & A. K. Aggarwal Nature 430, 377–380 (2004); Nair et al. reply . Human polymerase-ι belongs to the error-prone Y family of polymerases, which frequently incorporate incorrect nucleotides during DNA replication but can efficiently bypass DNA lesions1,2. On the basis of X-ray diffraction data, Nair et al. propose that Hoogsteen base-pairing is adopted by DNA during its replication by this enzyme3. Here I re-examine their X-ray data and find that the electron density is very weak for a Hoogsteen base pair formed between a template adenine deoxyribonucleotide in the syn conformation and a deoxythymidine 5′-triphosphate (dTTP), and that the fit is better for a normal Watson–Crick base pair. As a guanine–cytosine (G–C) base pair has no potential to form a Hoogsteen base pair at physiological pH, Hoogsteen base-pairing is unlikely to be used in replication by this polymerase.

Date: 2005
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/nature04199 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:437:y:2005:i:7057:d:10.1038_nature04199

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature04199

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().