The protein kinase A anchoring protein mAKAP coordinates two integrated cAMP effector pathways

Kimberly L. Dodge-Kafka, Joseph Soughayer, Genevieve C. Pare, Jennifer J. Carlisle Michel, Lorene K. Langeberg, Michael S. Kapiloff and John D. Scott ()
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Kimberly L. Dodge-Kafka: Howard Hughes Medical Institute, Vollum Institute
Joseph Soughayer: Howard Hughes Medical Institute, Vollum Institute
Genevieve C. Pare: Oregon Health and Sciences University
Jennifer J. Carlisle Michel: Howard Hughes Medical Institute, Vollum Institute
Lorene K. Langeberg: Howard Hughes Medical Institute, Vollum Institute
Michael S. Kapiloff: Oregon Health and Sciences University
John D. Scott: Howard Hughes Medical Institute, Vollum Institute

Nature, 2005, vol. 437, issue 7058, 574-578

Abstract: Abstract Cyclic adenosine 3′, 5′-monophosphate (cAMP) is a ubiquitous mediator of intracellular signalling events. It acts principally through stimulation of cAMP-dependent protein kinases (PKAs)1,2 but also activates certain ion channels and guanine nucleotide exchange factors (Epacs)3. Metabolism of cAMP is catalysed by phosphodiesterases (PDEs)4,5. Here we identify a cAMP-responsive signalling complex maintained by the muscle-specific A-kinase anchoring protein (mAKAP) that includes PKA, PDE4D3 and Epac1. These intermolecular interactions facilitate the dissemination of distinct cAMP signals through each effector protein. Anchored PKA stimulates PDE4D3 to reduce local cAMP concentrations, whereas an mAKAP-associated ERK5 kinase module suppresses PDE4D3. PDE4D3 also functions as an adaptor protein that recruits Epac1, an exchange factor for the small GTPase Rap1, to enable cAMP-dependent attenuation of ERK5. Pharmacological and molecular manipulations of the mAKAP complex show that anchored ERK5 can induce cardiomyocyte hypertrophy. Thus, two coupled cAMP-dependent feedback loops are coordinated within the context of the mAKAP complex, suggesting that local control of cAMP signalling by AKAP proteins is more intricate than previously appreciated.

Date: 2005
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DOI: 10.1038/nature03966

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