Insulin disrupts β-adrenergic signalling to protein kinase A in adipocytes

Jin Zhang, Christopher J. Hupfeld, Susan S. Taylor, Jerrold M. Olefsky and Roger Y. Tsien ()
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Jin Zhang: University of California at San Diego
Christopher J. Hupfeld: University of California at San Diego
Susan S. Taylor: University of California at San Diego
Jerrold M. Olefsky: University of California at San Diego
Roger Y. Tsien: University of California at San Diego

Nature, 2005, vol. 437, issue 7058, 569-573

Abstract: Abstract Hormones mobilize intracellular second messengers and initiate signalling cascades involving protein kinases and phosphatases, which are often spatially compartmentalized by anchoring proteins to increase signalling specificity1. These scaffold proteins may themselves be modulated by hormones2,3,4. In adipocytes, stimulation of β-adrenergic receptors increases cyclic AMP levels and activates protein kinase A (PKA)5, which stimulates lipolysis by phosphorylating hormone-sensitive lipase and perilipin6,7,8. Acute insulin treatment activates phosphodiesterase 3B, reduces cAMP levels and quenches β-adrenergic receptor signalling9. In contrast, chronic hyperinsulinaemic conditions (typical of type 2 diabetes) enhance β-adrenergic receptor-mediated cAMP production10. This amplification of cAMP signalling is paradoxical because it should enhance lipolysis, the opposite of the known short-term effect of hyperinsulinaemia. Here we show that in adipocytes, chronically high insulin levels inhibit β-adrenergic receptors (but not other cAMP-elevating stimuli) from activating PKA. We measured this using an improved fluorescent reporter and by phosphorylation of endogenous cAMP-response-element binding protein (CREB). Disruption of PKA scaffolding mimics the interference of insulin with β-adrenergic receptor signalling. Chronically high insulin levels may disrupt the close apposition of β-adrenergic receptors and PKA, identifying a new mechanism for crosstalk between heterologous signal transduction pathways.

Date: 2005
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DOI: 10.1038/nature04140

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