GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin

Miyako Ueguchi-Tanaka, Motoyuki Ashikari, Masatoshi Nakajima, Hironori Itoh, Etsuko Katoh, Masatomo Kobayashi, Teh-yuan Chow, Yue-ie C. Hsing, Hidemi Kitano, Isomaro Yamaguchi and Makoto Matsuoka ()
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Miyako Ueguchi-Tanaka: Nagoya University
Motoyuki Ashikari: Nagoya University
Masatoshi Nakajima: The University of Tokyo
Hironori Itoh: Nagoya University
Etsuko Katoh: National Institute of Agrobiological Sciences
Masatomo Kobayashi: BioResources Center
Teh-yuan Chow: Institute of Botany Academia, Sinica
Yue-ie C. Hsing: Institute of Botany Academia, Sinica
Hidemi Kitano: Nagoya University
Isomaro Yamaguchi: The University of Tokyo
Makoto Matsuoka: Nagoya University

Nature, 2005, vol. 437, issue 7059, 693-698

Abstract: Abstract Gibberellins (GAs) are phytohormones that are essential for many developmental processes in plants. It has been postulated that plants have both membrane-bound and soluble GA receptors; however, no GA receptors have yet been identified. Here we report the isolation and characterization of a new GA-insensitive dwarf mutant of rice, gid1. The GID1 gene encodes an unknown protein with similarity to the hormone-sensitive lipases, and we observed preferential localization of a GID1–green fluorescent protein (GFP) signal in nuclei. Recombinant glutathione S-transferase (GST)–GID1 had a high affinity only for biologically active GAs, whereas mutated GST–GID1 corresponding to three gid1 alleles had no GA-binding affinity. The dissociation constant for GA4 was estimated to be around 10-7 M, enough to account for the GA dependency of shoot elongation. Moreover, GID1 bound to SLR1, a rice DELLA protein, in a GA-dependent manner in yeast cells. GID1 overexpression resulted in a GA-hypersensitive phenotype. Together, our results indicate that GID1 is a soluble receptor mediating GA signalling in rice.

Date: 2005
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DOI: 10.1038/nature04028

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