Structure of the Sec13/31 COPII coat cage

Scott M. Stagg, Cemal Gürkan, Douglas M. Fowler, Paul LaPointe, Ted R. Foss, Clinton S. Potter, Bridget Carragher () and William E. Balch ()
Additional contact information
Scott M. Stagg: National Resource for Automated Molecular Microscopy
Cemal Gürkan: Departments of Cell
Douglas M. Fowler: Departments of Cell
Paul LaPointe: Departments of Cell
Ted R. Foss: Departments of Chemistry
Clinton S. Potter: National Resource for Automated Molecular Microscopy
Bridget Carragher: National Resource for Automated Molecular Microscopy
William E. Balch: Departments of Cell

Nature, 2006, vol. 439, issue 7073, 234-238

Abstract: Abstract Endomembranes of eukaryotic cells are dynamic structures that are in continuous communication through the activity of specialized cellular machineries1, such as the coat protein complex II (COPII), which mediates cargo export from the endoplasmic reticulum (ER)2,3. COPII consists of the Sar1 GTPase, Sec23 and Sec24 (Sec23/24), where Sec23 is a Sar1-specific GTPase-activating protein and Sec24 functions in cargo selection, and Sec13 and Sec31 (Sec13/31), which has a structural role3. Whereas recent results have shown that Sec23/24 and Sec13/31 can self-assemble to form COPII cage-like particles4, we now show that Sec13/31 can self-assemble to form minimal cages in the absence of Sec23/24. We present a three-dimensional reconstruction of these Sec13/31 cages at 30 Å resolution using cryo-electron microscopy and single particle analysis. These results reveal a novel cuboctahedron geometry with the potential to form a flexible lattice and to generate a diverse range of containers. Our data are consistent with a model for COPII coat complex assembly in which Sec23/24 has a non-structural role as a multivalent ligand localizing the self-assembly of Sec13/31 to form a cage lattice driving ER cargo export.

Date: 2006
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/nature04339 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:nature:v:439:y:2006:i:7073:d:10.1038_nature04339

Ordering information: This journal article can be ordered from
https://www.nature.com/

DOI: 10.1038/nature04339

Access Statistics for this article

Nature is currently edited by Magdalena Skipper

More articles in Nature from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().