Functional genomics reveals genes involved in protein secretion and Golgi organization

Frederic Bard, Laetitia Casano, Arrate Mallabiabarrena, Erin Wallace, Kota Saito, Hitoshi Kitayama, Gianni Guizzunti, Yue Hu, Franz Wendler, Ramanuj DasGupta, Norbert Perrimon and Vivek Malhotra ()
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Frederic Bard: University of California San Diego
Laetitia Casano: University of California San Diego
Arrate Mallabiabarrena: University of California San Diego
Erin Wallace: University of California San Diego
Kota Saito: University of California San Diego
Hitoshi Kitayama: University of California San Diego
Gianni Guizzunti: University of California San Diego
Yue Hu: University of California San Diego
Franz Wendler: the Ridgeway Mill Hill
Ramanuj DasGupta: Harvard Medical School
Norbert Perrimon: Harvard Medical School
Vivek Malhotra: University of California San Diego

Nature, 2006, vol. 439, issue 7076, 604-607

Abstract: Abstract Yeast genetics and in vitro biochemical analysis have identified numerous genes involved in protein secretion1,2. As compared with yeast, however, the metazoan secretory pathway is more complex and many mechanisms that regulate organization of the Golgi apparatus remain poorly characterized. We performed a genome-wide RNA-mediated interference screen in a Drosophila cell line to identify genes required for constitutive protein secretion. We then classified the genes on the basis of the effect of their depletion on organization of the Golgi membranes. Here we show that depletion of class A genes redistributes Golgi membranes into the endoplasmic reticulum, depletion of class B genes leads to Golgi fragmentation, depletion of class C genes leads to aggregation of Golgi membranes, and depletion of class D genes causes no obvious change. Of the 20 new gene products characterized so far, several localize to the Golgi membranes and the endoplasmic reticulum.

Date: 2006
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DOI: 10.1038/nature04377

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