STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis

Katrin I. Willig, Silvio O. Rizzoli, Volker Westphal, Reinhard Jahn () and Stefan W. Hell
Additional contact information
Katrin I. Willig: Departments of NanoBiophotonics
Silvio O. Rizzoli: Max Planck Institute for Biophysical Chemistry
Volker Westphal: Departments of NanoBiophotonics
Reinhard Jahn: Max Planck Institute for Biophysical Chemistry
Stefan W. Hell: Departments of NanoBiophotonics

Nature, 2006, vol. 440, issue 7086, 935-939

Abstract: Single-synapse microscopy STED (stimulated emission depletion) microscopy is an emergent light microscopy technique that overcomes the diffraction barrier limiting the resolution of conventional fluorescence microscopes. This brings structures the size of the synaptic vesicles (40 nm) into the picture. Now STED microscopy has been used to resolve individual synaptic vesicles in single synapses of the mammalian central nervous system for the first time. The synaptic vesicle recycling that is central to neurotransmitter release has been intensely studied for more than 30 years, but a major question has remained unanswered: do their components diffuse on the plasma membrane, or do they remain together? STED microscopy reveals that at least one main component, synaptotagmin-1, remains clustered after exocytosis and is recycled en bloc.

Date: 2006
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DOI: 10.1038/nature04592

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