Detecting transient intermediates in macromolecular binding by paramagnetic NMR

Junji Iwahara and G. Marius Clore ()
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Junji Iwahara: National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health
G. Marius Clore: National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health

Nature, 2006, vol. 440, issue 7088, 1227-1230

Abstract: Nuclear magnetic resonance spectroscopy was used to detect transient intermediates that occurred when the HOXD9 homeodomain was searching for its target sequence in a short DNA duplex, and determined that this transcription factor binds to non-cognate DNA sites to enhance the rate of specific association.

Date: 2006
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DOI: 10.1038/nature04673

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