S-Nitrosylated protein-disulphide isomerase links protein misfolding to neurodegeneration

Takashi Uehara, Tomohiro Nakamura, Dongdong Yao, Zhong-Qing Shi, Zezong Gu, Yuliang Ma, Eliezer Masliah, Yasuyuki Nomura and Stuart A. Lipton ()
Additional contact information
Takashi Uehara: Center for Neuroscience and Aging
Tomohiro Nakamura: Center for Neuroscience and Aging
Dongdong Yao: Center for Neuroscience and Aging
Zhong-Qing Shi: Center for Neuroscience and Aging
Zezong Gu: Center for Neuroscience and Aging
Yuliang Ma: Proteomic Facility, Burnham Institute for Medical Research
Eliezer Masliah: University of California at San Diego
Yasuyuki Nomura: Graduate School of Pharmaceutical Sciences, Hokkaido University
Stuart A. Lipton: Center for Neuroscience and Aging

Nature, 2006, vol. 441, issue 7092, 513-517

Abstract: Abstract Stress proteins located in the cytosol or endoplasmic reticulum (ER) maintain cell homeostasis and afford tolerance to severe insults1,2,3. In neurodegenerative diseases, several chaperones ameliorate the accumulation of misfolded proteins triggered by oxidative or nitrosative stress, or of mutated gene products4,5. Although severe ER stress can induce apoptosis2,6, the ER withstands relatively mild insults through the expression of stress proteins or chaperones such as glucose-regulated protein (GRP) and protein-disulphide isomerase (PDI), which assist in the maturation and transport of unfolded secretory proteins. PDI catalyses thiol–disulphide exchange, thus facilitating disulphide bond formation and rearrangement reactions7,8,9,10. PDI has two domains that function as independent active sites with homology to the small, redox-active protein thioredoxin7,8. During neurodegenerative disorders and cerebral ischaemia, the accumulation of immature and denatured proteins results in ER dysfunction11, but the upregulation of PDI represents an adaptive response to protect neuronal cells12,13,14. Here we show, in brains manifesting sporadic Parkinson's or Alzheimer's disease, that PDI is S-nitrosylated, a reaction transferring a nitric oxide (NO) group to a critical cysteine thiol to affect protein function15,16,17,18. NO-induced S-nitrosylation of PDI inhibits its enzymatic activity, leads to the accumulation of polyubiquitinated proteins, and activates the unfolded protein response. S-Nitrosylation also abrogates PDI-mediated attenuation of neuronal cell death triggered by ER stress, misfolded proteins or proteasome inhibition. Thus, PDI prevents neurotoxicity associated with ER stress and protein misfolding, but NO blocks this protective effect in neurodegenerative disorders through the S-nitrosylation of PDI.

Date: 2006
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DOI: 10.1038/nature04782

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